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. 2019 Jun;29(6):969–977. doi: 10.1101/gr.245399.118

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© 2019 Spektor et al.; Published by Cold Spring Harbor Laboratory Press

This article is distributed exclusively by Cold Spring Harbor Laboratory Press for the first six months after the full-issue publication date (see http://genome.cshlp.org/site/misc/terms.xhtml). After six months, it is available under a Creative Commons License (Attribution-NonCommercial 4.0 International), as described at http://creativecommons.org/licenses/by-nc/4.0/.

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Figure 1. — Overview of mATAC-seq. (A) Tn5 carrying methylated oligonucleotides (red and blue segments) is used to (B) perform tagmentation on nuclei at THS sites. (C) Tagmented DNA is end-repaired using 5mdCTP + dDTPs, purified, (D) bisulfite-converted, amplified, and (E) sequenced to measure DNA methylation and accessibility simultaneously. Sample data are shown for one region in HCT116 cells. Peak height in accessibility track is proportional to read abundance; bar height in methylation track is proportional to extent of methylation at CpGs.