FIG 3.

Hydrolysis of xylan and Xyl₆ by Xyn30A. Hydrolysis was carried out in a reaction mixture containing 10 μg ml⁻¹ purified Xyn30A and 10 mg ml⁻¹ birchwood glucuronoxylan (A), 10 mg ml⁻¹ wheat arabinoxylan (B), or 1 mM Xyl₆ in 50 mM sodium acetate (pH 4.0) (C). The reaction mixtures containing xylan and Xyl₆ were incubated at 40 and 45°C, respectively. Concentrations of xylose and Xyl₂ were determined by HPAEC-PAD.