FIG 6.

Functional pathogenicity determinants of JEONG5446. (A) Adherence of 11 representative isolates to Caco-2 cells. Assays of the adherence of isolates were conducted in three independent experiments at a multiplicity of infection (MOI) of 10 (10 bacterial cells to 1 Caco-2 cell). Bars indicate the mean values ± standard errors of the means (SEM), and asterisks (*) indicate statistical differences (P < 0.05) compared with the result for the negative control, DH5α. (B) Survival curves of JEONG5446, EDL933, and DH5α with (MMC) or without (not treated [NT]) mitomycin C treatment. Bacterial cell cultures were treated with mitomycin C when the OD₆₀₀ reached 0.5. (C) Dot blot analysis for detecting Shiga toxin type 1 (Stx1) and Stx2 in JEONG5446 treated with (+) or without (−) mitomycin C. EDL933 (Stx1 and Stx2 positive) and DH5α (Stx1 and Stx2 negative) were used as the positive and negative control, respectively.