. 2019 Jun 17;85(13):e00535-19. doi: 10.1128/AEM.00535-19

TABLE 1.

List of oligonucleotides used in the present study^a

Oligonucleotide	Sequence (5′–3′)	Purpose
MnoSFor	ATGGCCGAAGCGGCCCGCACC	Cloning of mnoS in pMS-QS-CHS and pSS4
MnoSRev	GCAGAATGTCGTTGAGACGGTTGAGC	Cloning of mnoS in pMS-QS-CHS and pSS4
MnoRFor	ATGACCGTCACGACGCGCGAG	Cloning of mnoS in pMS-QS-CHS and pSS4
MnoRRev	GATCAACCCGCGCTTGCTC	Cloning of mnoS in pMS-QS-CHS and pSS4
MnoSUpFor	CAGGTCGGGGGCCTGCTCGACC	Amplification of mnoS upstream fragment
MnoSUpRev	CCACGTACATCACCACAAGCACCCACTCGGCGTCGAGG	Amplification of mnoS upstream fragment
MnoSHygFor	CGAGTGGGTGCTTGTGGTGATGTACGTGGCGAACTCC	Amplification of Hyg^r cassette
MnoSHygRev	CGTACACGGCCTGATCCGGGGGGCGTCAGG	Amplification of Hyg^r cassette
MnoSDownFor	CCTGACGCCCCCCGGATCAGGCCGTGTACGCGGCGAGC	Amplification of mnoS downstream fragment
MnoSDownRev	GGTGTTCACCGGCGTCGTGCGTTCC	Amplification of mnoS downstream fragment
MnoSLongFor	CTGGCGTTCACCAACGCGATCC	PCR confirmation of ΔmnoS
MnoSLongRev	CGATCTGACCCCTGACGAACTGTCC	PCR confirmation of ΔmnoS
MnoRD60AFor	CGACGTGGTGCTGCTGGCCCTCAAGCTCTCGGCCGGATC	Site directed mutation for generating MnoR^D60A
6244Upfor	AGAACTCTAGAGGTGGGCGAGGGTGC	Amplification of MSMEG_6244 upstream fragment
6244UpRev	CGTACATCACCACGCTCGCGTGCCGCGTC	Amplification of MSMEG_6244 upstream fragment
6244HygFor	GCACGCGAGCGTGGTGATGTACGTGGCGAAC	Amplification of Hyg^r cassette
6244HygRev	CTAACCGCCTGAGGGATCCGGGGGGCGTC	Amplification of Hyg^r cassette
6244DownFor	GACGCCCCCCGGATCCCTCAGGCGGTTAG	Amplification of MSMEG_6244 downstream fragment
6244DownRev	GCTGCTGTTCGGGTTTGGGTCGTTC	Amplification of MSMEG_6244 downstream fragment
6244LongFor	CAGGTCCGGGCAGCTGACACCACGG	PCR confirmation of ΔMSMEG_6244
6244LongRev	GCTGTTGTCGGTATCGCCACAGCATTACC	PCR confirmation of ΔMSMEG_6244
rpoBRTFor	TCGATGTCACTGTCCTTCTCGGATC	RT-qPCR for rpoB expression
rpoBRTRev	GACCGTCTGGCTCTTGATCTC	RT-qPCR for rpoB expression
mnoRTFor	TCTGCTTGTTGGTGGACTTG	RT-qPCR for mno expression
mnoRTRev	GTCGAACCCCAAGGACTACA	RT-qPCR for mno expression
6239RTFor	GAAATCGTGTTCGGCATCGATTCG	RT-qPCR for MSMEG_6239 expression
6239RTRev	CTCCAGACCTGCGGGGTCACG	RT-qPCR for MSMEG_6239 expression
mnopSDRev	GCATGCCAATGGCCATTGGTTCACTCCTCGCTG	Cloning of 200-bp mno fragment upstream from start codon in pSD5b
mno250pSDFor	ACGACCATCTAGAGCCTGAGCGATC
NobiotinpSD	CCACTGCAGTGCATATGGAAGTGATTCC	PCR amplification of probe for EMSA
biotinpSD	Biotin-CCACTGCAGTGCATATGGAAGTGATTCC	PCR amplification of probe for EMSA
6239pSDrev	ACCGCATGCGGCGCGGACTCCACCTGC	Cloning of 200-bp MSMEG_6239 fragment upstream from start codon in pSD5b
6239200pSDFor	GTATGCGACAAGGTGGTCGTCG

Sequence of each oligonucleotide from 5′ to 3′ is given. The purpose of each oligonucleotide in the present study is also mentioned for easy reference.