FIG 7.

CR fluorescent signal in a cellulose knockout Nissle strain. Plasmid pL6FO, harboring an IPTG-inducible csgBACEFG operon, was used to induce curli production in 2 Nissle-derived strains, PBP5 and AIK2. PBP5 contains a knockout of the native curli operon csgBACDEFG, and AIK2 is PBP5 with a further knockout of the cellulose-producing operon bcsEFGRQABZC. Aside from a minor reduction in the peak curli production, removal of the cellulose operon did not influence the background CR signal. Error bars show standard deviation from experiments performed in duplicate.