TABLE 2.

Primers and probes used in melting curve genotyping analysis performed by hybridization probe or DNA sequencing for detecting mutations involved in reduced susceptibility to tetracyclines, spectinomycin, macrolides, lincosamides, and fluoroquinolones

Target mutation(s)	Primer or probe	Sequence (5′ to 3′)	Annealing temp (°C)	Amplicon size (bp)	Reference
rrs A965 and/or A967	Forward primer	GCA TAG GAA ATG ATG CTA CC	61	808	This study
	Reverse primer	TGC TCC ATG TCA CCA CTT C
	Hybridization probe	CGG TGG AGC ATG TGG TTT AA-FITC
		LC Red640-TTG AAG ATA CGC GTA GAA CC-phosphate
rrs C1192	Forward primer	Same as rrs A965 and/or A967	61	808	This study
	Reverse primer	Same as rrs A965 and/or A967
	Hybridization probe	CCG AGT AAT CGG GAG GAA GG-FITC
		LC Red640-GGG GAC GAC GTC AAA TCA TC-phosphate
rrl DII G748	Forward primer	AGC TTT TGG GAA GAA GCG	65	836	This study
	Reverse primer	TTA CAT TGT CGG CGC AAG G
	Hybridization probe	CCC TAA GTG GAG GGT CGA AC-FITC
		LC Red640-GTA GTA CAC TGA AAC GTG CC-phosphate
rrl DV A2059	Forward primer	GGA CTT TTG TCT GAA TTT GCC	61	609	This study
	Reverse primer	GTT GAC TCC ACT GCT ACT G
	Hybridization probe	GAA AAC GCT GGG TTC CCG CA-FITC
		LC Red640-CAA GAC GAA AAG ACC CCA TG-phosphate
QRDR in gyrA	gyrA-F primer	GACGAATCATCTAGCGAG	59	531	21
	gyrA-R primer	GCCTTCTAGCATCAAAGTAGC
QRDR in parC	parC-F primer	GAGCAACAGTTAAACGATTTG	59	488	21
	parC-R primer	GGCATAACAACTGGCTCTT