FIG 5.

Microbial taxa impacted by pasteurization as detected by PMA treatment. The fold change in the proportions of bacterial taxa between PMA-treated and untreated milk collected from equipment after pasteurization. Prior to the fold change calculations for each taxa (PMA-treated aliquot/untreated aliquot), sequence counts of 0 were replaced with 1. Unchanged OTU counts from each sample were analyzed using LefSe with PMA treatment set as class and the collection event (one PMA-treated aliquot and one untreated aliquot from each sample were compared) set as subclass. Pairwise comparisons were made only with subclasses of the same name. A significant difference (LDA effect size > 2, P < 0.005) between the PMA-treated and untreated aliquots for all four pieces of equipment is indicated by an asterisk.