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. Author manuscript; available in PMC: 2019 Jun 18.
Published in final edited form as: Metabolism. 2016 Sep 22;66:14–22. doi: 10.1016/j.metabol.2016.09.005

Figure 4. Western diet feeding resulted in myocardial interstitial fibrosis and oxidative stress that were prevented by volitional exercise.

Figure 4

A) Quantification of picrosirus red staining alongside representative images for both WD and WD+Ex groups reveals a significant increase in interstitial collagen deposition (pink color) in WD fed mice that was prevented by exercise. Quantification of myocardial fibrosis reveals a significant reduction in interstitial fibrosis with exercise (Dashed line represents average value of control diet fed mice. N = 5 for WD group and N = 6 for WD+Ex group. * denotes p <0.01 for WD compared to WD+Ex.) B) Quantification of 3-nitrotyrosine staining alongside representative images for both WD and WD+Ex groups shows marked accumulation of peroxynitrite, an oxidant species, (dark brown color) that was prevented by volitional exercise. Quantification of 3-NT staining by average gray scale intensities showed amelioration of oxidant stress with exercise in WD fed mice. (Dashed line represents average value of non-exercised control diet fed mice. N = 6 for WD group and N = 7 for WD+Ex group. * denotes p < 0.01 for WD compared to WD+Ex.)