Table 2.

Relative expression of transcripts (listed alphabetically) identified from whole skeletal muscle potentially affected by aging and/or exercise using a RT RT-PCR screening method

		Young		Elderly
Transcript, Accession No.	Ct	Pre	Post	Pre	Post
α-Cardiac actin (ACTC1), NM_005159	23.94	1.0	8.0	1.4	3.1
Atrogin-1 or F-box protein 32 (MAFbx), NM 058229	28.9	1.0	0.8	0.4	0.5
Ciliary neurotrophic factor (CNTF) NM 000614	28.18	1.0	1.2	0.5	0.7
Connective tissue growth factor (CTGF), NM_001901	27.04	1.0	0.6	0.6	0.5
Early growth response protein-1 (EGR1), NM 001964	26.99	1.0	1.8	0.6	0.6
Fibroblast growth factor-6 (FGF6), NM_020996	26.02	1.0	1.3	0.5	0.9
Fibronectin (FN1), X02761	25.57	1.0	0.6	0.1	0.3
Growth and differentiation factor 8 or myostatin (GDF8), NM_005259	25.54	1.0	0.5	0.5	0.4
Insulin-like growth factor binding protein-5 (IGFBP5), NM_000599	21.66	1.0	0.8	0.5	0.6
Insulin-like growth factor-1 (IGF1), NM_000618 ‘	27.71	1.0	0.6	0.3	0.4
Matrix metallopeptidase-2 (MMP2), NM_004530	25.06	1.0	1.3	0.5	0.8
Osteosarcoma viral oncogene homolog (c-fos), AY212879	29.33	1.0	0.9	0.3	0.4
TIMP metallopeptidase inhibitor-1 (TIMP1), X03124	27.83	1.0	2.4	0.9	1.4
Vascular endothelial growth factor-A (VEGFA), M32977	25.38	1.0	1.2	0.5	0.9
Vascular endothelial growth factor-D (VEGFD), NM_004469	28.26	1.0	0.9	0.5	0.6

Transcript name, abbreviation, and sequence are available using the provided accession number at http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?CMD=search&DB=gene. Ct is threshold real-time (RT) RT-PCR cycle for detection of transcript in young muscle pre-exercise pool. Transcript levels are presented as the fold-difference relative to the young muscle pre-exercise pool with differences ≤0.5 or ≥1.5 in boldface. Pre, pre-exercise; Post, post-exercise.