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. 2019 Jun 18;14(6):e0216285. doi: 10.1371/journal.pone.0216285

Fig 1. Identification of alternative splicing form of IGF-IIR: IGF-IIRα and development of IGF-IIRα transgenic rats.

Fig 1

(a) identification of novel IGF-IIRα mRNA transcripts contained intron9 (nt 645–806). Detection of mRNA transcripts by northern blots using random primed DNA labeling probe contained intron9 (nt 645–806) in various tissue samples, (b) the structural domain of IGF-IIR and IGF-IIRα, (c) full length of IGF-IIR and IGF-IIRα mRNA, (d) schematic diagram of IGF-IIRα transgenic vector construction. IGF-IIRα with N-terminal 3XHA, which is driven by cardiac specific promoter Myh6 promoter, (e) DNA genotyping was performed by PCR for TG-IGF-IIRα positive founders identification and (f) protein expression of TG-IGF-IIRα from the heart.