Table 1. Three-factor PERMANOVA results for differences in bacterial/archaeal and fungal community structure affected by three N fertilization levels (0, 100, and 200 kg N ha-1), two soil depths (0–5 and 5–15 cm), and three sampling seasons following N input (Spring, Summer, and Fall 2017).
| Source | df | SS | MS | PS-F | P(perm) | UP | Estimate | Sq.root |
|---|---|---|---|---|---|---|---|---|
| Bacteria/Archaea | ||||||||
| N | 2 | 4010 | 2005 | 1.44 | 0.0384 | 9873 | 12.9 | 3.6 |
| Depth | 1 | 21742 | 21742 | 15.68 | 0.0001 | 9886 | 282.7 | 16.8 |
| Season | 2 | 31772 | 15886 | 11.46 | 0.0001 | 9884 | 302.1 | 17.3 |
| N×Depth | 2 | 1825 | 913 | 0.66 | 0.9879 | 9869 | -19.7 | -4.4 |
| N×Season | 4 | 5071 | 1268 | 0.91 | 0.6992 | 9809 | -7.4 | -2.7 |
| Depth×Season | 2 | 9614 | 4807 | 3.47 | 0.0001 | 9793 | 142.5 | 11.9 |
| N×Depth×Season | 4 | 3729 | 932 | 0.67 | 0.9995 | 9805 | -56.8 | -7.5 |
| Res | 126 | 1.8E+05 | 1386 | |||||
| Total | 143 | 2.5E+05 | ||||||
| Fungi | ||||||||
| N | 2 | 8995 | 4497 | 1.29 | 0.0319 | 9808 | 21.5 | 4.6 |
| Depth | 1 | 14453 | 14453 | 4.17 | 0.0001 | 9859 | 152.6 | 12.4 |
| Season | 2 | 54894 | 27447 | 7.92 | 0.0001 | 9808 | 499.6 | 22.4 |
| N×Depth | 2 | 3897 | 1949 | 0.56 | 1.0000 | 9803 | -63.2 | -8.0 |
| N×Season | 4 | 14744 | 3686 | 1.06 | 0.3351 | 9720 | 13.8 | 3.7 |
| Depth×Season | 2 | 19295 | 9647 | 2.78 | 0.0001 | 9793 | 257.6 | 16.1 |
| N×Depth×Season | 4 | 9534 | 2383 | 0.69 | 1.0000 | 9704 | -135.2 | -11.6 |
| Res | 126 | 4.4E+05 | 3465 | |||||
| Total | 143 | 5.6E+05 |
df, degrees of freedom; SS, sum of squares; MS, mean squares; PS‐F, pseudo‐F value; P(perm), permutation P-value based on 9999 permutations; UP, unique values of test statistic obtained under permutation; Estimate, estimated component of variation; Sq.root, square root of the estimated component of variation.