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. 2019 Jun 18;14(6):e0216679. doi: 10.1371/journal.pone.0216679

Fig 12. Characterization of a dromedary camel POMC prohormone.

Fig 12

(A) Translated protein sequence, predicted signal peptide shown in lowercase letters, confirmed cleavage sites shown in green, confirmed amidation sites shown in blue, peptides detected by MALDI TOF MS underlined. (B) Phylogenetic tree for POMC prohormones from human (P01189), cow (P01190), ship (P01191) and a novel camel sequence that all share 75% identity. This tree was produced using CLUSTALO pairwise alignments and neighbor joining method. Camel POMC shares 89.4% identity with sheep, 88.3% with cow, and 79.3% with human POMC prohormones. Representative MALDI TOF mass spectra of individual camel pituitary extracts. (C) Full mass range spectrum from a summer sample, peaks matching the masses of predicted peptides are labelled (Table 1); (D) Zoom-in view showing differential detection of neurotensin, melanotropin alpha, α-MSH, and its post-translationally modified forms depending on the season. Labels: W, winter; S, summer.