Table 1. Peptide mass fingerprint assignment of peptides in the camel neurointermediate lobe extracts as determined by MALDI-TOF MS.
| Label | Sequence | (M+H)theor | (M+H)exp* | Accuracy | Prohormone |
|---|---|---|---|---|---|
| a | FRWGKPVa | 888.52 | 888.52 | 3 | POMC |
| b | ac-GDGAEPGPREa | 1025.47 | 1025.63 | -159 | POMC |
| c | HFRWGKPVa | 1025.58 | -47 | POMC | |
| d | cYFQNcPRGa | 1084.45 | 1084.54 | -85 | AVP |
| e | HFRWGSPPKD | 1226.61 | 1226.62 | -9 | POMC |
| f | MEHFRWGKPVa | 1285.66 | 1285.67 | -4 | POMC |
| g | MQHFRWGSPPKD | 1485.71 | 1485.74 | -21 | POMC |
| h | AGAPEPAEHAQPGVY | 1493.70 | 1493.74 | -24 | AVP |
| i | GAGPGPRGDGAEPGPREa | 1575.76 | 1575.8 | -22 | POMC |
| j | des-ac-SYSMEHFRWGKPVa | 1622.79 | 1622.82 | -18 | POMC |
| k | ac-SYSMEHFRWGKPVa | 1664.80 | 1664.83 | -16 | POMC |
| l | di-ac-YSMEHFRWGKPVa | 1706.82 | 1706.82 | 0 | POMC |
| m | pGlu-LAGERPEAALGPEAPAE | 1788.88 | 1788.88 | 0 | POMC |
| n | RPVKVYPNGVEDESAE | 1788.88 | 0 | POMC | |
| o | VAAGAGPGPRGDGAEPGPREa | 1816.91 | 1816.91 | 0 | POMC |
| p | VQLAGAPEPAEHAQPGVY | 1833.92 | 1833.92 | 0 | AVP |
| q | DGGPYKMQHFRWGSPP | 1859.87 | 1859.92 | -26 | POMC |
| r | RPVKVYPNGVEDESAEA | 1859.92 | -2 | POMC | |
| s | RPVKVYPNGVEDESAEAF | 2006.98 | 2006.95 | 17 | POMC |
| t | DGGPYKMQHFRWGSPPKD | 2102.99 | 2102.96 | 14 | POMC |
| u | RPVKVYPNGVEDESAEAFPLE | 2346.16 | 2346.13 | 14 | POMC |
| v | ELAGERPEAALGPEAPAEGATAQAE | 2435.17 | 2435.14 | 13 | POMC |
Monoisotopic theoretical mass computed from the amino acid sequence of protonated molecular ion (M+H); *Mean (M+H) computed from all neurointermediate lobe samples where predicted mass has been experimentally detected; Accuracy of detection is represented as parts per million, ppm; PTMs are italicized lower case letters: a-amidation, ac-acetylation, c-half disulfide bond, pGlu-pyroglutamic acid. POMC: proopiomelanocortin. AVP: arginine vasopressin; Peptides labelled m/n and q/r could not be distinguished and thus both sequences are given.