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. Author manuscript; available in PMC: 2020 Jun 17.
Published in final edited form as: Curr Biol. 2019 May 30;29(12):1954–1962.e4. doi: 10.1016/j.cub.2019.04.073

Figure 1. Cop1 mediates the cryptochromes-dependent repression of canonical GR target genes.

Figure 1.

(A) Left, qPCR analysis of cDNAs prepared from DKO MEFs infected with lentiviruses expressing untagged human Cry1 under the control of a doxycycline-inducible promoter before and after dexamethasone treatment. Right, corresponding cell extracts were analyzed by immunoblotting.

(B) The experiment was performed as in (A), except that inducible human Cry2 was expressed in DKO MEFs.

(C) The experiment was performed as in (A), except that cells were treated with an siRNA oligo to Cop1 or a non-targeting siRNA oligo (NT).

(D) The experiment was performed as in (B), except that cells were treated with an siRNA oligo to Cop1 or a non-targeting siRNA oligo (NT).

(E) Left, qPCR analysis of cDNAs prepared from wild-type MEFs, before and after KL001 treatment and in presence or absence of dexamethasone treatment. Right, corresponding cell extracts were analyzed by immunoblotting.

(F) Left, qPCR analysis of cDNAs prepared from wild-type MEFs, before and after KL001 treatment, after dexamethasone treatment and in presence or absence of Cop1 knockdown. Right, corresponding cell extracts were analyzed by immunoblotting. The asterisk indicates a cross-reacting band.