(A) Mice were retro-orbitally injected with Adeno-associated viruses expressing either scramble shRNA or Cop1 shRNA. After 4 weeks, mice were tail-injected with dexamethasone for 1 hour and then sacrificed at ZT2 and ZT10. Liver tissue was then collected, and RNA was extracted and analyzed by qPCR.
(B) Mice were retro-orbitally injected with Adeno-associated viruses expressing either scramble shRNA or Cop1 shRNA. After 4 weeks, primary hepatocytes were isolated and treated with KL001 followed by glucagon treatment. Hepatocytes were then collected, and RNA was extracted and analyzed by qPCR. See also Figure S1.