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. Author manuscript; available in PMC: 2020 Jun 17.
Published in final edited form as: Curr Biol. 2019 May 30;29(12):1954–1962.e4. doi: 10.1016/j.cub.2019.04.073

Figure 3. Cop1 mediates the cryptochromes-dependent rhythmic repression of the GR transcriptional activity in vivo and the repression of the glucagon receptor signaling.

Figure 3.

(A) Mice were retro-orbitally injected with Adeno-associated viruses expressing either scramble shRNA or Cop1 shRNA. After 4 weeks, mice were tail-injected with dexamethasone for 1 hour and then sacrificed at ZT2 and ZT10. Liver tissue was then collected, and RNA was extracted and analyzed by qPCR.

(B) Mice were retro-orbitally injected with Adeno-associated viruses expressing either scramble shRNA or Cop1 shRNA. After 4 weeks, primary hepatocytes were isolated and treated with KL001 followed by glucagon treatment. Hepatocytes were then collected, and RNA was extracted and analyzed by qPCR. See also Figure S1.