KEY RESOURCES TABLE
| REAGENT or RESOURCE | SOURCE | IDENTIFIER |
|---|---|---|
| Antibodies | ||
| Rabbit anti-Cop1, 1:2000 | Bethyl | Cat# A300-894A, RRID:AB_625290 |
| Rabbit anti-Cry1, 1:2000 | Bethyl | Cat# A302-614A, RRID:AB_10555376 |
| Rabbit anti-c-Jun, 1:5000 | Cell Signaling Technology | Cat# 9165, RRID:AB_2130165 |
| Rabbit anti-Skp1, 1:5000 | Michele Pagano laboratory | N/A |
| Rabbit anti-Cry2, 1:2000 Human-Cry2 detection |
Bethyl | Cat# A302-615A, RRID:AB_10554665 |
| Rabbit anti-Cry2, 1:1000 Endogenous mouse-Cry2 detection |
Proteintech Group | Cat# 13997-1-AP, RRID:AB_10860100 |
| Rabbit anti-JunB, 1:1000 | Bethyl | Cat# A302-704A, RRID:AB_10749029 |
| Mouse anti-β-Actin 1:5000 | Sigma-Aldrich | Cat# A5441, RRID:AB_476744 |
| Rabbit anti-Ets-1, 1:2000 | Cell signaling | Cat# 14069, N/A |
| Rabbit anti-p53, 1:2000 | Santa Cruz Biotechnology | Cat# sc-6243, RRID:AB_653753 |
| Rabbit anti-FLAG, 1:5000 | Sigma-Aldrich | Cat# F7425, RRID:AB_439687 |
| Mouse anti-Stat3, 1:2000 | Santa Cruz Biotechnology | Cat# sc-8019, RRID:AB_628293 |
| Mouse anti-Mta1, 1:1000 | Santa Cruz Biotechnology | Cat# sc-17773, RRID:AB_627969 |
| Mouse anti-Atgl, 1:2000 | Santa Cruz Biotechnology | Cat# sc-365278, RRID:AB_10859044 |
| Rabbit anti-Ddb1, 1:5000 | Bethyl | Cat# A300-462A, RRID:AB_420928 |
| Rabbit anti-HA, 1:5000 | Bethyl | Cat# A190-108A, RRID:AB_67465 |
| Rabbit anti-Per2, 1:2000 | Bethyl | Cat# A303-109A, RRID:AB_10892917 |
| Rabbit anti-Clock, 1:2000 | Bethyl | Cat# A302-618A, RRID:AB_10555233 |
| Rabbit anti-Bmal1, 1:2000 | Bethyl | Cat# A302-616A, RRID:AB_10555918 |
| Rabbit anti-Fbxl3, 1:1000 | Signalway | Cat# 21459-1, RRID:AB_10760331 |
| Mouse anti-Det1, 1:500 | Santa Cruz Biotechnology | Cat# sc-514348, N/A |
| Rabbit anti-Cul4A, 1:2000 | Bethyl | Cat# A300-739A, RRID:AB_533380 |
| Rabbit anti-Dda1, 1:2000 | Proteintech Group | Cat# 14995-1-AP, RRID:AB_10859095 |
| Secondary HRP-conjugated antibodies | Thermo Fisher Scientific | |
| Chemicals, Peptides, and Recombinant Proteins | ||
| MG-132 (10 uM) | Peptides International | Cat# IZL-3175-v |
| Doxycycline (100 ng/mL) | Sigma-Aldrich | Cat# D9891 |
| ABsolute Blue qPCR SYBR Green Mix | Thermo Fisher Scientific | Cat# AB-4166B |
| Cycloheximide (20mg/mL) | Sigma-Aldrich | Cat# C7698 |
| Polybrene (8 mg/mL) | Sigma-Aldrich | Cat# TR-1003 |
| Puromycin | Sigma-Aldrich | Cat# P9620 |
| RNAi Max (Lipofectamine) | Thermo Fisher Scientific | Cat# 13778-500 |
| SiLentFect | Biorad | Cat# 1703360 |
| KL001 (10 μM) | Sigma | Cat# SML1032 |
| Critical Commercial Assays | ||
| RNA to cDNA EcoDry Premix (Random Hexamers) | Clontech/ Takara Bio | Cat# 639546 |
| QuikChange Site-directed Mutagenesis kit | Agilent | Cat# 200523 |
| Deposited Data | ||
| RNA sequencing data | GEO | GSE124388 |
| Raw images of western blots | This paper, Mendeley database | doi:10.17632/9ydg4zjb76.1 |
| Experimental Models: Cell Lines | ||
| Human: HEK-293T | ATCC | Cat# CRL-3216, RRID:CVCL_0063 |
| Human: U-2OS | ATCC | Cat# HTB-96, RRID:CVCL_0042 |
| Mouse: Cry1−/−Cry2−/− MEFs | Gift: Dr. Choogon Lee | N/A |
| Mouse: WT MEFs | Gift: Dr. Choogon Lee | N/A |
| Mouse: Beta-TC-6 | ATCC | Cat# CRL-11506, RRID:CVCL_0605 |
| Mouse: c-Jun−/− MEFs | Gift: Dr. Michael Karin | N/A |
| Experimental Models: Organisms/Strains | ||
| Mice: C57BL/6J, male | Jackson Laboratory | Cat# JAX:000664 IMSR_JAX:000664 |
| Oligonucleotides | ||
| siRNA: RFWD2 | Dharmacon | Cat# L-043438-01 |
| siRNA: Non-Targeting | Dharmacon | Cat# D-001810-01 |
| Quantitative PCR primer sequences for cell culture experiments | Table S1 | N/A |
| Quantitative PCR primer sequences for mouse liver tissue experiments | Table S2 | N/A |
| Recombinant DNA | ||
| Plasmid: pfmh-hCry1 | Addgene | Cat# 25843, RRID: Addgene_25843 |
| Plasmid: pSO2002-Cry2 | Addgene | Cat# #25842, RRID:Addgene_25842 |
| Plasmid: pcDNA4/TO/myc-His-Det1 | Abgent | Cat# DC09517 |
| Plasmid: pcDNA3-c-Jun | Michele Pagano laboratory | N/A |
| Plasmid: pcDNA3-Rfwd2 | Michele Pagano laboratory | N/A |
| pTRIPZ | Dharmacon | N/A |
| pBabe | Cell Biolabs | N/A |
| pcDNA3.1 | Life Technologies | N/A |
| Software and Algorithms | ||
| Prism software (version 5.0.3) | Graphpad Software, Inc | www.graphpad.com |
| STAR (version 2.4.5a) | ||
| bcl2fastq Conversion software (v.1.8.4) | ||
| FastQ Screen (v0.5.2) | ||
| featureCounts (Subread package v1.4.6-p3) | ||
| DESeq2 package (Bioconductor v3.3.0) | R statistical programming environment | |
| ‘heatmap.2’ within the package ‘gplots’ | R statistical programming environment | |
| Homer script “findMotifs.pl” | PMID: 20513432 | |
| Jalview version 2 | PMID: 19151095 | |