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. Author manuscript; available in PMC: 2020 Jul 1.
Published in final edited form as: J Immunol. 2019 May 10;203(1):282–292. doi: 10.4049/jimmunol.1801068

Figure 1. Feeding acetate and butyrate promotes mucosal IgA and systemic IgG responses in mice orally immunized with OVA and CT.

Figure 1.

Groups (n = 4–5) of WT mice were fed with or without antibiotics for 10 days. The antibiotic-treated and untreated WT mice were immunized with 100 μg OVA and 10 μg CT on day 0 and day 14 by gavage. The mice were fed with or without a mixture of 200 mM acetate (C2) and butyrate (C4) in drinking water for 28 days. Fecal pellets and serum samples were collected for analysis of OVA-specific IgA in feces (A), OVA-specific IgG in serum (B), CTb-specific IgA in feces (C), and CTb-specific IgG in serum (D) production by ELISA. One representative of three independent experiments was shown. The data were expressed as mean ± s.e.m. *p < 0.05, **p < 0.01.