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. Author manuscript; available in PMC: 2020 Jul 1.
Published in final edited form as: J Immunol. 2019 May 24;203(1):158–166. doi: 10.4049/jimmunol.1800976

Figure 3. Splenic macrophages from NOD mice inadequately transpresent and upregulate IL-15.

Figure 3.

A) Baseline IL-15 transpresentation, as measured by relative surface IL-15 Receptor alpha expression (IL-15Ra), is significantly reduced on splenic resident NOD macrophages (MΦs). B) Splenic resident macrophages from NOD mice fail to upregulate IL-15Ra expression to the same extent as B6 macrophages when stimulated with 10ug of the TLR3 agonist PolyI:C. Isotype control staining for IL-15Ra on B6 and NOD Macrophages is shown by the dotted line. Percent upregulation of IL-15Ra is calculated by first subtracting the isotype MFI from the individual mouse MFIs for each strain. Data represent one experiment, independently repeated three times with similar results. Data shown includes N=5 B6 mice and N=5 NOD mice. **p<0.01, by two-way ANOVA followed by Bonferroni post-test or student’s t-test.