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. Author manuscript; available in PMC: 2020 Jul 1.
Published in final edited form as: J Immunol. 2019 May 17;203(1):178–187. doi: 10.4049/jimmunol.1801657

Figure 6. DNA-PKcsPQR/PQR cells are moderately sensitive to radiation.

Figure 6

(A) The sensitivity to ionizing radiation (1, 2, 5 Gy) of DNA-PKcs+/+, DNA-PKcs−/−, DNA-PKcsPQR/PQR, and DNA-PKcsSD/SD primary MEFs. The relative survival represents the relative cellularity at the given dose as a proportion of the un-irradiated sample of the same genotype. The data represent the average and standard error of at least 5 independent wells per genotype per dose. The radiation sensitivity of DNA-PKcsPQR/PQR MEFs is statistically significantly different from both DNA-PKcs+/+ and DNA-PKcs−/− MEFs at all three doses tested (p<0.001). (B) Relative fold of growth (by cell counts) for DNA-PKcs+/+ and DNA-PKcsPQR/PQR splenic B cells during class switch recombination (4 days). The bars mark the means and standard errors. The unpaired student’s t-test p-value is 0.13. (C) The sensitivity to ionizing radiation (1, 2, 5 Gy) of DNA-PKcs+/+ and DNA-PKcsPQR/PQR B cells activated for CSR. Two independent pairs of DNA-PKcs+/+ and DNA-PKcsPQR/PQR B cells were assayed in two independent experiments (another is shown in Supplementary Figure 2E). DNA-PKcsPQR/PQR B cells are significantly more sensitive to radiation than the DNA-PKcs+/+ controls at all three radiation doses (p<0.05).