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. Author manuscript; available in PMC: 2020 Jun 1.
Published in final edited form as: Circ Genom Precis Med. 2019 May 17;12(6):e002403. doi: 10.1161/CIRCGEN.118.002403

Figure 6.

Figure 6.

Confirmation of CXCL6 as a mediator of hCPC function. (A) High expression levels of CXCL6 and CXCL8 in “GOOD” vs “POOR” pediatric patients. Statistical modeling demonstrated high expression levels of CXCL6 and CXCL8 mRNA in GOOD pediatric CPCs. Patients above threshold (dotted lines) are GOOD samples with significantly high expression of CXCL6 (top panel) and CXCL8 (bottom panel). (B) Correlation of CXCL6 and CXCL8 mRNAs with proliferation and migration functions in pediatric patients. CXCL6 and CXCL8 mRNA expression showed relatively high positive correlation with both proliferation and migration functions. Signals are the mean TMM values from edgeR. They have centered with respect to the overall mean gene expression of each sample (A and B). (C) Regression analysis of observed migration responses with protein expression levels of CXCL8 measured by ELISA in neonatal patients. Observed proliferation or migration responses for each neonate patient were plotted against CXCL8 protein expression levels. Observed migration responses in neonatal patients showed high correlation with CXCL8 protein expression levels (R2=0.74). (D). Comparison of in vitro proliferation and migration responses and protein expression levels of CXCL8 measured by ELISA. The abundance of CXCL8 protein levels (black bars) matched the migration induction potential of neonatal CPCs (gray bars). Expression level of CXCL8 protein did not fully match with the proliferation ability of the cells (white bars). Proliferation assay was not performed with neonatal #1050 cell lines. This cell line was lost after collecting the conditioned media for migration assay. (E) Comparison of the abundance of CXCL8 proteins measured with ELISA and predicted expression levels of CXCL8 mRNA in neonatal CPCs. Predicted richness of CXCL8 mRNA (white bars) and the observed protein level of CXCL8 expressed in neonatal CPCs (black bars) matched very closely and showed an average accuracy of 82%. ND=not detected. CXCL8 protein expression (ELISA) and observed proliferation and migration responses are normalized to the maximum signal.