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Kinetics analysis of Tris conjugation with 2-FPBA/APBA. A) Associative relaxation of 2-FPBA and Tris at 2 mM concentration. B) Associative relaxation of 2-APBA and Tris at 8 mM concentration. C) Dissociation kinetics of 2-FPBA-Tris conjugate monitored by monitoring absorption at 254 nm. D) Dissociation kinetics of 2-APBA-Tris conjugate monitored by monitoring absorption at 254 nm. For C and D, the conjugates were diluted to 100 µM to initiate the experiments.
