Table 1.

Summary of human mtDNA and nDNA restriction endonuclease fragment sizes detected via Southern blotting with mtDNA- and nDNA-specific probes.

	mtDNA fragment sizes		nDNA fragment sizes
Single or double digest reactionsa	Observed molecular weight (kb)b	Expected molecular weight (kb)	Observed molecular weight (kb)b	Expected molecular weight (kb)
SphI	15.9 ± 0.78c	16.6	N/Ad	29.7
BamHI	15.9 ± 0.78c	16.6	2.10 ± 0.08	2.2
PstI	15.4 ± 0.62c	14.5	6.05 ± 0.13	5.5
Mono. EcoRI	8.65 ± 0.57	8.1	6.30 ± 0.08	5.8
Poly. EcoRI	N/A	N/A	16.4 ± 0.98c	17.8
Mono. EcoRI + SphI	6.75 ± 0.10	6.4	6.05 ± 0.10	5.6
Poly. EcoRI + SphI	N/A	N/A	16.6 ± 0.93c	17.5
Mono. EcoRI + PstI	8.65 ± 0.57	8.1	5.23 ± 0.13	4.8
Poly. EcoRI + PstI	N/A	N/A	6.03 ± 0.10	5.5

^aMono. EcoRI and Poly. EcoRI, the 18S monomorphic and polymorphic EcoRI alleles respectively that are discussed in the legend to Fig. 2.

^bThe average values for n = 4 blots done on different days using 5 preparations of whole-cell extracted DNA are reported with errors as standard deviations.

^cFor SphI, BamHI, PstI, Poly. EcoRI, and Poly. EcoRI + SphI digests generating fragment lengths greater than 10 kb, restriction fragment lengths detected via Southern blotting were estimated using linear regression of the log₁₀ base pair values of Lambda DNA/HindIII Marker, 2 fragments loaded onto the same gel versus distance traveled in millimeters. For the remaining reactions generating fragments lengths less than 10 kb, the log₁₀ base pair values of exACTGene™ 1kb Plus DNA ladder fragments versus distance traveled in millimeters were utilized as standards to estimate molecular weights.

^dThe molecular weight of the 18S nDNA fragment cut with SphI exceeded the range of both standard curves generated utilizing either the lambda DNA HindIII fragments or the exACTGene™ ladder fragments; therefore, a calculated molecular weight is not reported for this sample. N/A, not applicable.