Skip to main content
. Author manuscript; available in PMC: 2020 Jul 1.
Published in final edited form as: Gastroenterology. 2019 Mar 11;157(1):193–209.e9. doi: 10.1053/j.gastro.2019.03.013

Figure 5. Cyclic stretch utilizes integrins to activate the Notch pathway.

Figure 5.

(A) RNA was isolated from primary murine LSECs that were subjected to cyclic stretch. RNA-seq revealed significant upregulation of genes related to integrin signaling. (B) RGD-peptide prevents stretch-induced upregulation of CXCL1 (ANOVA P≤0.05), Hes1 (ANOVA P≤0.05) and Hey1 (ANOVA P≤0.05). (C) Protein expression of Hes1 is higher in HUVEC subjected to cyclic stretch compared with unstretched controls. Quantification was performed using ImageJ with fold change represented in the adjacent graph. (D) Notch1 agonist Jagged-1 upregulates CXCL1 mRNA levels. (E) HUVEC transfected with an siRNA against the Notch1 receptor have lower mRNA levels of CXCL1 after cyclic stretch compared with HUVEC transfected with siControl (ANOVA P≤0.05). siRNA knockdown of Notch is shown in adjacent panel. (F) Treatment of HUVEC with the Notch inhibitor DAPT decreases mRNA expression of CXCL1 (ANOVA P≤0.05) (n=3–5; *P≤0.05 for all panels).