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. Author manuscript; available in PMC: 2020 Jul 1.
Published in final edited form as: J Immunol. 2019 May 20;203(1):105–116. doi: 10.4049/jimmunol.1801032

Figure 3.

Figure 3

A) mRNA expression of Nfatc1-Gapdh ratios in male BMM cultures from WT and PAR1 KO mice treated with M-CSF and RANKL (30 ng/ml for both) for 2, 3 or 5 days. N = 3 for each group. B) Cytoplasmic and nuclear p65 protein in 3 replicate male BMM cultures from WT and PAR1 KO mice. Cells were cultured with M-CSF and RANKL (30 ng/ml for both) for 3 days to stimulate PAR1 expression in WT cells and then transferred to serum free media for 3 hours before being challenged with RANKL for 15 minutes. Cytoplasmic and nuclear fractions were isolated and run in western blot assay. Control protein blots were for actin in the cytoplasmic fraction and TATA box binding protein (TBP) in the nuclear fraction. Lower graphs are the ratio of p65 to actin band density in the cytoplasmic fraction and p65 to TBP band density in the nuclear fraction.