Figure 4.

ICV-Injected Aβ-Th1 Cells Exhibit Reduced Effector Functions in 5XFAD/MHCII^−/− Mice

5XFAD and 5XFAD/MHCII^−/− mice were ICV-injected with either Aβ-Th1 T cells or PBS (n = 4–5 mice per group), killed at 21 dpi, and their brains collected and analyzed with IHC, qPCR, or flow cytometry.

(A) Representative IHC images showing Aβ plaques co-localized with GFP+ T cells in brain sections derived from 5XFAD/MHCII^−/− mice at 21 dpi and immunolabeled with anti-Aβ (red) and anti-CD4 (green). The right image is a 3D reconstruction of z-sections (22.5 μm overall, 0.75 μm/slice) of the framed area. Scale bars, 30 μm (left) and 10 μm (right).

(B) The quantitative analysis (right graph) of Aβ plaque load per volume of the cortical section in 5XFAD/MHCII^−/− mice ICV-injected with either PBS or Th1 T cells (right) was performed with IMARIS. Bars represent means ± SEM.

(C) qPCR analysis of IFN-γ and the chemokines CXCL9, CXCL10, and CXCL11 in the different groups of mice. Bars represent means ± SEM.

(D) Flow cytometry plots demonstrating the gating strategy for myeloid cells (CD45+ CD11b+; middle) and presumed infiltrated macrophages (Ly6C+ CX3CR1−; right).

(E) The frequency of CX3CR1−Ly6C+ cells in all groups. Bars represent means ± SEM of one experiment out of two performed. Each symbol represents an individual mouse. *p < 0.05, **p < 0.01, ***p < 0.001 (one-way ANOVA).