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. 2019 Jun 12;7:130. doi: 10.3389/fbioe.2019.00130

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Copyright © 2019 Lenzen, Wynands, Otto, Bolzenius, Mennicken, Blank and Wierckx.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Modulation of expression of genomically integrated RtPAL, RsTAL, aroG^fbr, and tyrA^fbr. Increasing promoter strengths are listed from left to right. Cells were cultivated in biological triplicates in MSM containing 20 mM glucose as a sole carbon source at 30°C and 300 rpm in System Duetz plates, expression of ferulic genes was induced with 0.2 mM IPTG. Titers of final samples (96 h) are shown. Error bars indicate standard error of the mean.