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. 2019 Jun 12;7:130. doi: 10.3389/fbioe.2019.00130

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Copyright © 2019 Lenzen, Wynands, Otto, Bolzenius, Mennicken, Blank and Wierckx.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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4-hydroxybenzoate production from glycerol and xylose by engineered P. taiwanensis VLB120 strains. Cells were grown in biological triplicates in MSM containing glycerol or 24 mM xylose as a sole carbon source in 24-well System Duetz plates at 30°C and 300 rpm. Expression of ferulic genes was induced with 0.2 mM IPTG. Cultivations of (A) strain CL3 and (B) CL3.3 Values of biomass (circles), glycerol (squares), 4-hydroxybenzoate (triangles), and trans-cinnamate (inverted triangles) are shown. (C) Aromatics concentrations and biomass after 96 h. The used substrate concentrations are indicated on the X-axis. For P. taiwanensis VLB120 CL3.3 and CL4.3, expression of ppsA-pgi was induced with 0.1 mM salicylate. Error bars indicate standard error of the mean.