Table 1.
Strains used or engineered in this study.
| Strain | Characteristics | References |
|---|---|---|
| E. coli PIR1 | F−Δlac169 rpoS(Am) robA1 creC510 hsdR514 endA recA1uidA(ΔMluI)::pir-116; host for oriV(R6K) vectors in high copy number | Thermo Fisher Scientific |
| E. coli DH5α λpir | λpir lysogeny of DH5α | Glover and Hames, 1985 |
| E. coli DH5α pTNS1 | supE44 ΔlacU169 (φ80 lacZΔM15) hsdR17 (rk- mk+) recA1 endA1 gyrA96 thi-1 relA1 | de Lorenzo lab |
| E. coli HB101 pRK2013 | Helper strain carrying plasmid pRK2013; F−λ−hsdS20(rB− mB−) recA13 leuB6(Am) araC14 Δ(gpt-proA)62 lacY1 galK2(Oc) xyl-5 mtl-1 thiE1 rpsL20(SmR) glnX44(AS) | Figurski and Helinski, 1979 |
| E. coli DH5α pBBFLP | Strain used for excision of kanamycin resistance cassette F−endA1 glnV44 thi-1 recA1 relA1 gyrA96 deoR nupG purB20 φ80dlacZΔM15 Δ(lacZYA-argF)U169, hsdR17(rK−mK+), λ− |
de las Heras et al., 2008 |
| P. taiwanensis VLB120 | Wild type | Panke et al., 1998 |
|
P. taiwanensis VLB120 CL1 |
Derived from P. taiwanensis VLB120; Deficient for pobA, hpd; carrying genes ech, vdh and fcs randomly integrated via Tn5 transposon. |
This study |
|
P. taiwanensis VLB120 CL1 RtPAL |
P. taiwanensis VLB120 CL1 carrying plasmid pJT'RtPAL | This study |
|
P. taiwanensis VLB120 CL1 FjTAL |
P. taiwanensis VLB120 CL1 carrying plasmid pJT'FjTAL | This study |
|
P. taiwanensis VLB120 CL1 TcXAL |
P. taiwanensis VLB120 CL1 carrying plasmid pJT'TcXAL | This study |
|
P. taiwanensis VLB120 CL1 RsTAL |
P. taiwanensis VLB120 CL1 carrying plasmid pJT'RsTAL | This study |
|
P. taiwanensis VLB120 CL1.1 RtPAL |
Derived from P. taiwanensis VLB120 CL1; Deficient for quiC, quiC1, quiC2; carrying plasmid pJT'RtPAL |
This study |
|
P. taiwanensis VLB120 CL2 |
Derived from P. taiwanensis VLB120 CL1.1; Point mutation in trpE resulting in P290S substitution |
This study |
|
P. taiwanensis VLB120 CL2 RtPAL |
P. taiwanensis VLB120 CL2 carrying plasmid pJT'RtPAL | This study |
|
P. taiwanensis VLB120 CL2 RtPALa |
P. taiwanensis VLB120 CL2 carrying plasmid pJT'RtPALa | This study |
|
P. taiwanensis VLB120 CL2 RtPALat |
P. taiwanensis VLB120 CL2 carrying plasmid pJT'RtPALat | This study |
|
P. taiwanensis VLB120 CL2 RtPALatt |
P. taiwanensis VLB120 CL2 carrying plasmid pJT'RtPALatt | This study |
|
P. taiwanensis VLB120 CL2 BG14a RtPALat |
P. taiwanensis VLB120 CL2 carrying genes RtPAL, aroGfbr, tyrAfbr at attTn7 site under control of 14a promoter |
This study |
|
P. taiwanensis VLB120 CL2 BG14d RtPALat |
P. taiwanensis VLB120 CL2 carrying genes RtPAL, aroGfbr, tyrAfbr at attTn7 site under control of 14d promoter |
This study |
|
P. taiwanensis VLB120 CL2 BG14e RtPALat |
P. taiwanensis VLB120 CL2 carrying genes RtPAL, aroGfbr, tyrAfbr at attTn7 site under control of 14e promoter |
This study |
|
P. taiwanensis VLB120 CL3 |
P. taiwanensis VLB120 CL2 carrying genes RtPAL, aroGfbr, tyrAfbr at attTn7 site under control of 14g promoter |
This study |
|
P. taiwanensis VLB120 CL2 BG14fg RtPALat |
P. taiwanensis VLB120 CL2 carrying genes RtPAL, aroGfbr, tyrAfbr at attTn7 site under control of 14fg promoter |
This study |
|
P. taiwanensis VLB120 CL2 BG14ffg RtPALat |
P. taiwanensis VLB120 CL2 carrying genes RtPAL, aroGfbr, tyrAfbr at attTn7 site under control of 14ffg promoter |
This study |
|
P. taiwanensis VLB120 CL3.1 |
P. taiwanensis VLB120 CL3 carrying plasmid pBNT'ppsA | This study |
|
P. taiwanensis VLB120 CL3.2 |
P. taiwanensis VLB120 CL3 carrying plasmid pBNT'pgi | This study |
|
P. taiwanensis VLB120 CL3.3 |
P. taiwanensis VLB120 CL3 carrying plasmid pBNT'ppsA-pgi | This study |
|
P. taiwanensis VLB120 CL4 |
Derived from P. taiwanensis VLB120 CL4; Carrying genes RsTAL, aroGfbr, tyrAfbr at attTn7 site under control of 14f promoter |
This study |
|
P. taiwanensis VLB120 CL4.3 |
P. taiwanensis VLB120 CL4 carrying plasmid pBNT'ppsA-pgi | This study |