Table 2.
Plasmids used and constructed in this work.
| Plasmid | Characteristics | References |
|---|---|---|
| pJT'mcs | AmpR GmR, expression vector, under control of Ptac promoter | Nijkamp et al., 2007 |
| pTacPAL | AmpR GmR, expression vector, harboring RtPAL under control of Ptac promoter | Nijkamp et al., 2007 |
| pBELK | Mini-Tn5 delivery vector; tnpA oriV(R6Kγ) oriT(RK2) lacIQ Ptrc bla FRT-aphA-FRT, AmpR KmR | Nikel and de Lorenzo, 2013 |
| pBELK ferulic s | Mini-Tn5 delivery vector harboring genes ech, vdh and fcs from P. putida S12 | This study |
| pJT'RsTAL | pJT expression vector harboring RsTAL | This study |
| pJT'FjTAL | pJT expression vector harboring FjTAL | This study |
| pJT'TcXAL | pJT expression vector harboring TcXAL | This study |
| pJT RtPAL | pJT expression vector harboring RtPAL | This study |
| pBWatt | pSEVA234 expression vector harboring genes aroGfbr, tyrAfbr, tktA; KmR, ori pBBR1, laqIq-Ptrc | This study |
| pJT'RtPALa | pJT expression vector harboring gene aroGfbr | This study |
| pJT'RtPALat | pJT expression vector harboring gene aroGfbr, tyrAfbr | This study |
| pJT'RtPALatt | pJT expression vector harboring gene aroGfbr, tyrAfbr, tktA | This study |
| pBG14a | Tn7 delivery vector; KmR GmR, ori R6K, Tn7L, and Tn7R flanks, BCD2–msfgfp fusion, promoter no. 28 | Zobel et al., 2015 |
| pBG14d | Tn7 delivery vector; KmR GmR, ori R6K, Tn7L, and Tn7R flanks, BCD2–msfgfp fusion, promoter no. 51 | Zobel et al., 2015 |
| pBG14e | Tn7 delivery vector; KmR GmR, ori R6K, Tn7L, and Tn7R flanks, BCD2–msfgfp fusion, promoter no. 17 | Zobel et al., 2015 |
| pBG14f | Tn7 delivery vector; KmR GmR, ori R6K, Tn7L, and Tn7R flanks, BCD2–msfgfp fusion, promoter no. 25 | Zobel et al., 2015 |
| pBG14g | Tn7 delivery vector; KmR GmR, ori R6K, Tn7L, and Tn7R flanks, BCD2–msfgfp fusion, promoter no. 42 | Zobel et al., 2015 |
| pBG14fg | Tn7 delivery vector; KmR GmR, ori R6K, Tn7L, and Tn7R flanks, BCD2–msfgfp fusion, hybrid promoter composed of No. 25 & No. 42 | Köbbing et al. in preparation |
| pBG14ffg | Tn7 delivery vector; KmR GmR, ori R6K, Tn7L, and Tn7R flanks, BCD2–msfgfp fusion, hybrid promoter 2 x No. 25 & No. 42 | Köbbing et al. in preparation |
| pBG14a RtPALat | Tn7 delivery vector harboring genes RtPAL, aroGfbr, tyrAfbr, promoter no. 28 | This study |
| pBG14d RtPALat | Tn7 delivery vector harboring genes RtPAL, aroGfbr, tyrAfbr, promoter no. 51 | This study |
| pBG14e RtPALat | Tn7 delivery vector harboring genes RtPAL, aroGfbr, tyrAfbr, promoter no. 17 | This study |
| pBG14g RtPALat | Tn7 delivery vector harboring genes RtPAL, aroGfbr, tyrAfbr, promoter no. 42 | This study |
| pBG14fg RtPALat | Tn7 delivery vector harboring genes RtPAL, aroGfbr, tyrAfbr, hybrid promoter no. 25 & no. 42 | This study |
| pBG14ffg RtPALat | Tn7 delivery vector harboring genes RtPAL, aroGfbr, tyrAfbr, hybrid promoter 2 × no. 25 & no. 42 | This study |
| pBG14f RsTALat | Tn7 delivery vector harboring genes RsTAL, aroGfbr, tyrAfbr, promoter no. 25 | This study |
| pRK2013 | Helper plasmid for conjugational transfer; KmR, oriV(RK2/ColE1), mob+ tra+ |
Figurski and Helinski, 1979 |
| pTNS1 | Helper plasmid; AmpR, ori R6K, TnSABC+D operon | Choi et al., 2005 |
| pSW-2 | GmR, oriRK2, xylS, Pm → I-sceI (transcriptional fusion of I-sceI to Pm) | Martínez-García and de Lorenzo, 2011 |
| pBBFLP | Helper plasmid for excision of antibiotic markers, oriV(pBBR1), oriT(RK2) RK2 mob+ λPR::FLP λ(cI857) sacB tet, TetR | de las Heras et al., 2008 |
| pEMG pobA | Knockout vector carrying TS1 and TS2 flanking regions of p-hydroxybenzoic acid hydroxylase (pobA) | Wynands et al., 2018 |
| pEMG hpd | Knockout vector carrying TS1 and TS2 flanking regions of 4-hydroxyphenylpyruvate dioxygenase (hpd) | Wynands et al., 2018 |
| pEMG quiC | Knockout vector carrying TS1 and TS2 flanking regions of dehydroshikimate dehydratase (quiC) | Wynands et al., 2018 |
| pEMG quiC1 | Knockout vector carrying TS1 and TS2 flanking regions of dehydroshikimate dehydratase (quiC1) | Wynands et al., 2018 |
| pEMGu quiC2 | Knockout vector carrying TS1 and TS2 flanking regions dehydroshikimate dehydratase (quiC2) | Wynands et al., 2018 |
| pEMGu trpEP290S | Knockout vector carrying TS1 and TS2 flanking regions of the codon for the P290S substitution of trpE gene | Wynands et al., 2018 |
| pBNT'mcs | Expression vector harboring salicylate-inducible nagR/PNagAa promoter, KmR | Verhoef et al., 2010 |
| pBNT'ppsA | pBNT expression vector harboring gene encoding phosphoenolpyruvate synthase (ppsA) | This study |
| pBNT'pgi | pBNT expression vector harboring gene encoding glucose-6-phosphate isomerase (pgi) | This study |
| pBNT'ppsA-pgi | pBNT expression vector harboring gene encoding phosphoenolpyruvate synthase (ppsA), and glucose-6-phosphate isomerase (pgi) | This study |