Figure 2.

Expression Profiles of Zic Transcription Factors in ESCs and EpiLCs

(A) qRT-PCR analysis of Zic2, Zic3, Zic5, and Otx2 expression in the indicated cell states (n = 3).

(B) Western blot analysis of ZIC3, OTX2, and ERK2 expression. The asterisk marks a non-specific band. Quantification of ZIC3 protein levels is shown below each lane (n = 3).

(C) The scRNA-seq analysis of Zic2, Zic3, Zic5, and Otx2 expression. t-Distributed Stochastic Neighbor Embedding (t-SNE) analysis of the entire scRNA-seq dataset is shown on the right, with the originating cell types color coded. d1EpiLCs are circled.

See also Figure S6.