Stochastic noise in splicing machinery |
Humans |
Computational analysis |
Most AS is a consequence of stochastic noise in the splicing machinery, and has no functional significance |
Melamud and Moult, 2009 |
Assessing the contribution of alternative splicing to proteome diversity |
Plants |
Computational analysis |
AS contributes to transcriptome diversity but its contribution to protein diversity is limited |
Severing et al., 2009 |
Isoform-specific recruitment to polyribosomes |
Humans |
Frac-Seq |
Addition to translation AS plays role in sequestration and mRNA-decay |
Sterne-Weiler et al., 2013 |
Assess the role of AS in proteome diversity |
Humans |
Computational analysis |
Most genes have a single dominant isoform at the protein level, whereas homologous exons have important cellular roles |
Abascal et al., 2015 |
Expression of protein coding gene isoforms at protein level |
Humans |
Computational analysis |
Most highly expressed gene have single dominant isoform represented at the protein level |
Ezkurdia et al., 2015 |
Ribosomal-engaged landscape of AS transcripts |
Humans and mouse |
Ribo-Seq |
Majority of splice variants are translated into proteins |
Weatheritt et al., 2016 |
Tunable protein synthesis by transcript isoforms |
Humans |
TrIP-Seq |
Alternatively spliced isoform levels effects translation output |
Floor and Doudna, 2016 |
AS mediated expansion of protein interaction capabilities |
Humans |
ORF-Seq and PPI |
Large number of alternative isoforms in the human proteome are “functional alloforms” |
Yang et al., 2016 |
Transcriptome survey and contribution of AS toward proteome diversity |
Plants |
RNA-Seq and Ribo-Seq |
AS increases protein complexity, however, its contribution is lower in plants as compared to humans |
Yu et al., 2016 |
Impact of AS on human proteome |
Humans |
RNA-Seq and SWATH-MS |
IR reduces the protein diversity but fine-tunes the human proteome functionality |
Liu et al., 2017 |
Relationship between AS and protein complexity |
Humans |
Computational analysis |
Majority of alternatively spliced transcripts may not be translated into proteins |
Tress et al., 2017a |
AS contribution in transcriptome and proteome diversity in Physcomitrella patens
|
Plants |
RNA-Seq and qRT-PCR |
AS has a small effect on proteome diversity but shapes the transcriptome |
Fesenko et al., 2017 |