Figure 5.

The Autonomic Nervous System Regulates Lymphatic Contractility In Vivo

(A) Schematic overview of in vivo imaging of flank collector contractility.

(B) Representative example of measured NIR-dye intensity in the flank collector over time; topical treatment was done at time point 2 min.

(C–E) Effects of topical treatment with neural agonists and antagonists on lymphatic contraction frequency (C), amplitude (D), and pumping score (E). Shown are the post-treatment values as a percentage of pretreatment values for mice under injection anesthesia (ketamine, xylazine, and acepromazine).

(G–I) Same is shown for mice under isoflurane: changes in frequency (G), amplitude (H) and pumping score (I).

(F and J) Quantification of the tonic contraction of the flank collector upon treatment with neural agonists and antagonists under injection anesthesia (F; ketamine, xylazine, acepromazine) and isoflurane (J).

(K and L) Isoproterenol-mediated phosphorylation of eNOS, as assessed (K) and quantified (L) by western blot. VEGF-A was used as positive control. The western blot shown is a representative example of at least three independent experiments.

^∗p < 0.05, ^∗∗p ≤ 0.01. Data are presented as mean ± SD. (C–F) n = 8 vessels for PBS, and n = 6 vessels for all other treatments. (G–J) n = 4 vessels per treatment. (K and L) n = 1. See also Figure S5.