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. 2019 Jun 11;27(11):3305–3314.e13. doi: 10.1016/j.celrep.2019.05.050

Figure 6.

Figure 6

Tumor-Draining Lymphatic Collectors Contract More Actively

(A) Growth of intradermally injected B16F10 tumors was measured over time using a caliper (n = 7 mice).

(B) Overview picture of the flank area in a naive (left) and a tumor-bearing Prox1-GFP mouse (right). In the vicinity of the flank collector (asterisk), additional lymphatic collectors were formed draining the tumor.

(C) Compared to physiological LVs (left), tumor-draining vessels show denser innervation, visualized with the pan-neural marker TuJ1 (Prox1-GFP staining, green; αSMA, yellow).

(D) Quantification of innervation density (nerve length/vessel length).

(E) Tumor-draining LVs show higher baseline activity than the respective vessels in non-tumor-bearing mice with regard to frequency, amplitude, and pumping score.

(F) Contractility of tumor-draining vessels can be inhibited by atropine, phentolamine, and isoproterenol.

∗p < 0.05, ∗∗p ≤ 0.01, and ∗∗∗p ≤ 0.001. Scale bars represent 500 μm (B) and 25 μm (C). Data represent mean ± SD, and dots in (D) and (E) represent vessels. (F) n = 4 vessels per treatment. BV, blood vessel. See also Figure S6.