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. 2019 Jun 12;9:495. doi: 10.3389/fonc.2019.00495

Figure 2.

Figure 2

FOSL2 is targeted by miR-597-5p in colon cells. (A) Schematic representation of complementarity of FOSL2 3′ UTR and miR-597-5p seed sequence. (B) Relative Renilla luciferase activity of constructs with wild type 3′ UTR of FOSL2 or its mutant (with deletion of miR-597-5p binding site) in FHC and LoVo cells. (C) QRT-PCR analysis of miR-597-5p in FHC and LoVo cells transfected with miR-597-5p antagomir and mimic, respectively. (D) Relative Renilla luciferase activity of constructs with wild type or mutant FOSL2 3′ UTR of in FHC and LoVo cells, alone or in combination with anti-miR-597-5p antagomir or miR-597-5p mimic, respectively. All data in (B–D) are expressed as mean ± SD of three independent experiments. *P < 0.05; NS, non-significant.