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. 2019 Jun 12;9:495. doi: 10.3389/fonc.2019.00495

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Copyright © 2019 Li, Liu, Fang, Wang and Fei.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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MiR-597-5p replenishment in the LoVo cells downregulates mesenchymal cell markers. (A) Immunoblot analysis to determine the effect of miR-597-5p replenishment in LoVo cells and inhibition in the FHC cells on FOSL2 and epithelial (E-Cadherin) and mesenchymal cell (Fibronectin and Vimentin) markers. Following stripping of blots, GAPDH was used to probe for equal loading of samples. From among three independent experiments, one representative blot is shown here. (B) Representative immunofluorescence images of E-cadherin and Vimentin in LoVo cells and FHC cells, mock transfected or co-transfected with miR-597-5p mimic and antagomir, respectively. Scale bar, 30 μm.