Ectopic crystal formation and ectopic expression of collagen I. A, Articular bones of mice fed the LPM or NPM. Left set of panels, light micrograph of sectioned and stained cartilage with hematoxylin & eosin. The set to the right was polarized light micrograph. Scale bars, 200 μm. B, Articular bones of mice fed the LPM or NPM were immunostained with collagen I (middle panels) and collagen II–specific antibodies (bottom panels). Left set of panels, NPM. Right set of panels, LPM. Upper panels, hematoxylin & eosin staining. Middle and lower panels, the enlarged images of the boxes in the upper images. Scale bars, 50 μm. C, The expression levels of collagen I and collagen II mRNA in articular bones of mice fed the LPM (n = 3) or NPM (n = 3). Values are mean ± SE.