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Detection of cytokines in serum and milk. A, The multiplex suspension arrays of mice fed with LPD or NPD. Left panel, concentration of tumor necrosis factor α (TNFα) (n = 3). Middle panel, concentration of interleukin-1α (IL-1α) (n = 3). Right panel, concentration of IL-6 (n = 3). Values are mean ± SE. B, Western blot analysis with IL-6 antibody in the milk of mice fed the LPD or NPD. The expression of GAPDH served as an internal control. C, Scheme of experiment.
