Fig. 2.

Detection of undifferentiated hiPSCs in hiPSC-derived cardiomyocytes using the LIN28/qRT-PCR method. (A) Schematic diagram of culture procedure for cardiomyocyte. (B) qRT-PCR analysis of cardiomyocyte markers, TNNT2, GATA4, NKX2.5 and MYH6. Total RNA was isolated from hiPSC-derived cardiomyocytes (black bar) and primary cardiomyocytes (white bar). The mRNA levels are shown relative to those in primary cardiomyocytes. (C) Flow cytometry analysis of TNNT2 in hiPSCs (red), and hiPSC-derived cardiomyocytes at day 20 (blue). (D) LIN28 expression in hiPSCs differentiating into cardiomyocytes (d10 and d20). LIN28 mRNA levels are shown relative to that in undifferentiated hiPSCs. hCM: human cardiomyocyte. The expression levels of target genes were normalized to those of the GAPDH transcript. Results are presented as the mean ± standard deviation (n = 3).