Fig. 4.

Detection of LIN28 mRNA in various types of human tissues using the LIN28/ddPCR method. (A) Absolute quantification of LIN28 mRNA copy number in normal tissues using ddPCR. Total RNA (50 ng) was analyzed. (B) qRT-PCR analysis of normal tissues. Total RNA (50 ng) was analyzed. LIN28, NANOG, OCT3/4 and SOX2 mRNA levels are shown as expression relative to those in undifferentiated hiPSCs. The expression levels of target genes were normalized to those of the ribosomal RNA. Results are presented as the mean ± standard deviation of three different lots of each tissue.