Fig. 1.

Comparison of growth effects between FBS and PRP in canine BM-MSC. Canine primary BM-MSC were cultured with media containing 10% FBS (FBS+) or 10% PRP (PRP+). After the first passage, MSC grown in FBS+ were plated on 6 well at a density of 3 × 10⁴/well. Then half of the 6 wells were grown in FBS+ (a, b), and the rest were grown in PRP+ (c, d). BM-MSC cultured in PRP + had proliferated faster than in FBS+ 6 days later (b, d). After the first passage, MSC grown in PRP+ were plated and cultured in FBS+ (e, f) or in PRP+ (g, h). Similar results were obtained 8 days later (f, h). All phase contrast digital images were taken every other day with an Olympus IX71 microscope. Scale bar = 200 μm.