Fig. 5.

Canine BM-MSC transplantation into ischemic limb. Left iliac artery of each male beagle (n = 4) was ligated and dissected to induce lower leg ischemia. Their own BM-MSC were cultured with 10% PRP, labeled with DiI, and then injected into the left quadriceps muscle of thigh at 10 sites with 100μl aliquots (n = 3). Only PBS was injected into the same sites of the control animal (n = 1). The animal implanted with 9.0 × 10⁶ cells was sacrificed 2 weeks after operation (a–d) and the animal implanted with 6.0 × 10⁶ cells was sacrificed 6 weeks after operation (e–h). (b, f): H&E staining of left quadriceps muscle of thigh. (a, c, d, e, g, h):Fluorescent micrographs after immunostaining for vWF (green). DiI (red), DAPI (blue). DiI positive transplanted cells were not overlapped with vWF positive endothelial cells. All images were taken with a Keyence BIOREVO fluorescence microscope.