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. 2019 May 30;16:283–297. doi: 10.1016/j.isci.2019.05.040

Figure 1.

Figure 1

Assessment of LifeAct-GFP Levels in Lymphocyte Subsets

(A) Flow cytometry analysis of B, CD4, and CD8 cells' LifeAct-GFP expression levels from spleen, LN, and blood. Statistical analysis comparing cell subsets obtained from LN or blood with the corresponding cell type obtained from spleen.

(B) Flow cytometry results showing levels of LifeAct-GFP expression on B1a, B1b, and B2 cells in the peritoneum.

(C) Detailed flow cytometric analysis of LifeAct-GFP levels of splenic lymphocyte subsets (T0, T1, T2, FO, MZP, MZ, and GC cells). For the right graph, T1, T2, FO MZP MZ, and GC cells are compared with T0. For the left graph, B1, B2a, and B1b cells are compared with (B).

(D) Representative flow cytometry plots showing LifeAct-GFP expression on FO B cells compared with different lymphocyte subsets of interest. Mean fluorescence data are shown as mean ± SEM of the relative expression of LifeAct-GFP (A–C). Negative controls had an average mean fluorescence intensity of 240.

Data are representative of three experiments in triplicates. Error bars show mean ± SEM. *p < 0.05, **p < 0.01, ***p < 0.001. Data analysis performed with Prism analysis of variance (ANOVA).