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. 2015 Nov 26;2:32–41. doi: 10.1016/j.reth.2015.10.001

Fig. 1.

Fig. 1

Map of the pTCF4-CMVpro-GL4.20 plasmid. Three copies of the optimal TCF-4 motif CCTTTGATC and cytomegalovirus (CMV) promoter were cloned from the Tcf4-CMVpro-Luc plasmid [9] and inserted at BglII and HindIII restriction enzyme sites in the multiple cloning sites of pGL4.20 luciferase reporter plasmid (Promega Corp., Fitchburg, WI), designated pTCF4-CMVpro-GL4.2. pTCF-CMVpro-GL4.20 was linearized by Pst1 and electroporated into UE7T-13 cells to establish cell lines for the assessment of Wnt/β-catenin activity in UE7T-13 cells.