Supplemental Fig. 2.

Differentiation potential of SEES-1, SEES-2, and SEES-3 cells A) Histological analysis of teratomas containing multidifferentiated tissues derived from SEES-1, SEES-2, and SEES-3 cells. Pigmented epithelium (ectoderm), cartilage (mesoderm), and gut epithelial tissues (endoderm). Scale bars are 200 μm. B) SEES-1, SEES-2, and SEES-3 cells that were differentiated in vivo via EB formation expressed markers of the primary germ layers, ectoderm (TUJ1), mesoderm (cTnT∗), and endoderm (AFP). ∗cTnT: cardiac troponin T, a cardiac marker. SEES-1 and SEES-2 cells: scale bars are 200 μm for TUJ1 and 50 μm for cTnT and AFP. SEES-3 cells: scale bars are 100 μm for TUJ1 and 50 μm for cTnT and AFP.