Skip to main content
. 2015 Feb 19;1:18–29. doi: 10.1016/j.reth.2014.12.004

Fig. 3.

Fig. 3

Derivation of xenogeneic-free hESCs on the hMSC feeder layer Under completely xenogeneic-free conditions, four hESC lines were derived from the inactivated hMSC feeder layer using the laser ablation system. Typical ESC morphology was readily visible. Alkaline phosphatase (ALP) activity was detected in each SEES cell line. Chromosome analysis of SEES-4, SEES-5, SEES-6, and SEES-7 cells showed normal karyotypes: 46,XX, 46,XX, 46,XY, and 46,XX, respectively.