Fig. 5.

Differentiation of three germ layers of xenogeneic-free SEES cell lines A) SEES cells differentiated in vitro via EBs expressed markers of the primary germ layers. Immunohistochemical analyses of markers of the ectoderm (TUJ1), mesoderm (αSMA), and endoderm (AFP) layers are shown. SEES-4: scale bars are 100 μm; SEES-5: scale bars are 200 μm for TUJ1 and 100 μm for αSMA and AFP; SEES-6: scale bars are 100 μm for TUJ1 and αSMA and 200 μm for AFP; SEES-7: scale bars are 100 μm. B) SEES cells differentiated in vivo via teratoma formation. Hematoxylin and eosin staining revealed germ layer derivatives, such as neural tissues, pigmented epithelium (ectoderm), cartilage (mesoderm), and gut epithelial tissues (endoderm). Scale bars are 200 μm.