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(A–B) BrdU incorporation into cardiomyocytes under each culture condition as measured by FACS (N = 3, **P < 0.01). (C–E) Immocytochemical identification of cardiomyocytes in two-chamber culture dishes on Day 1 and Day 5 by confocal microscopy; YFP (green), cTnT (red), Hoechst 33258 (blue nuclei), scale bar = 500 μm. YFP(+)/cTnT(+) cell number at Day 1 was set to 1.0 (N = 4, **P < 0.01, *P < 0.05).
