Figure 3.

Ets21c Drives Epithelial Renewal by Triggering EC Apoptosis

(A–D) Representative confocal images of 6- and 20-day-old control posterior midguts (A and C) and those expressing ets21c^RNAi (B and D) in ECs (Myo1A^TS) marked by GFP. Immunostaining labels cell membranes (Arm). DAPI labels nuclei.

(E) Diameter measurements of 10-day-old posterior midguts expressing ets21c^WT and ets21c^RNAi in ECs (Myo1A^TS) (n = 18–22).

(F–H) Representative confocal images of 10-day-old control posterior midguts (F) and those overexpressing ets21c^RNAi (G) and ets21c^WT (H) in ECs (Myo1A^TS). Immunostaining labels activated caspase Dcp1, cell membranes (Arm), and EEs (Pros).

(I–K) Representative confocal images of 6-day-old control posterior midguts (I) and those overexpressing ets21c^RNAi (J) and ets21c^WT (K) in ECs (Myo1A^TS) marked by GFP. Images show single confocal sections of epithelial cross-sections with the gut lumen oriented to the bottom. Immunostaining labels cell membranes (Arm). DAPI labels nuclei.

(L and M) Representative confocal images of 6-day-old posterior midguts overexpressing ets21c^WT alone (L) or in combination with p35 (M) in ECs (Myo1A^TS) marked by GFP. Immunostaining labels cell membranes (Arm). DAPI stains nuclei.

(N) Quantification of pH3⁺ cells per midgut overexpressing ets21c^WT and p35 in ECs (Myo1A^TS) (n = 20–28).

(O and P) Representative confocal images of recovery experiments showing posterior midguts of flies overexpressing ets21c^WT in ECs (Myo1A^TS) marked by GFP for 3 days at a permissive temperature (29°C) (O) compared to those shifted back to a restrictive temperature (18°C) for 3 additional days (P).

Data represent means (SDs); ^∗∗∗p < 0.001; n.s. = non-significant. Scale bars: 50 μm.

See also Figure S2.