Figure 5.

Cell-Type-Specific Sets of Target Genes Mediate the Cellular Responses to Ets21c

(A–E) Representative confocal images of 6-day-old control posterior midguts (A) and those overexpressing ets21c^WT alone (B) or in combination with RNAi-transgenes against pvf1 (C), upd3 (D), and imp (E) in ISCs/EBs (esg^TS) marked by GFP.

(F–J) Representative confocal images of 6-day-old control posterior midguts (F) and those overexpressing ets21c^WT alone (G) or in combination with RNAi-transgenes against pvf1 (H), upd3 (I), and eip93F (J) in ECs (Myo1A^TS) marked by GFP.

(K–O) Representative confocal images of 10-day-old control posterior midguts (K) and those overexpressing ets21c^WT alone (L) or in combination with RNAi-transgenes against pvf1 (M), upd3 (N), and eip93F (O) in ECs (Myo1A^TS). Immunostaining labels activated caspase Dcp1.

(P) Quantification of pH3⁺ cells per midgut overexpressing ets21c^WT and RNAi-transgenes against pvf1, upd3, imp, and eip93F in ISCs/EBs (esg^TS) or ECs (Myo1A^TS) (n = 10–27). Data represent means (SDs); ^∗∗∗p < 0.001.

(Q) Schematic representation of Ets21c functions in the Drosophila adult intestine.

DAPI labels nuclei. Scale bars: 50 μm.

See also Figure S4.