Figure 6.

Loss of ets21c Prolongs Lifespan but Renders Flies Sensitive to Stress

(A–D) Representative confocal images of 6- and 30-day-old w¹¹¹⁸ (A and C) and ets21c^Δ10 posterior midguts (B and D). ISCs/EBs are labeled with an esg-GFP^YB0232 protein-trap reporter. Arrows indicate cells with strong (ISC/EB, blue), weak (pre-EC, green), or no (EC/EE, gray) esg-GFP^YB0232 signal in aging midguts. Immunostainings label cell membranes (Arm) and EEs (Pros). DAPI stains nuclei.

(E) Quantification of cells per confocal image field that shows strong (ISC/EB, blue), weak (pre-EC, green), or no (EC/EE, gray) esg-GFP^YB0232 expression in 30-day-old w¹¹¹⁸ and ets21c^Δ10 posterior midguts. Data represent means (SDs); n = 13–17; ^∗∗∗p < 0.0001.

(F) Percentage of survival of w¹¹¹⁸ (n = 200) and homozygous ets21c^Δ10 (n = 314) adult females over time (mean difference of 4 days).

(G) Percentage of survival of w¹¹¹⁸ (n = 100) and homozygous ets21c^Δ10 (n = 120) adult females fed with 5 mM PQ (mean difference of 52 h) or mock solution (w¹¹¹⁸ n = 40; ets21c^Δ10 n = 60).

(H) Percentage of survival of adult females overexpressing ets21c^WT (n = 60; mean difference of 36 h) and ets21c^RNAi (n = 80; mean difference of 31 h) in ISCs/EBs (esg^TS) compared to control flies fed with PQ (n = 80) or mock solution (n = 80).

(I) Percentage of survival of adult females overexpressing ets21c^WT (n = 80; mean difference of 31 h) and ets21c^RNAi (n = 80; mean difference of 23 h) in ECs (Myo1A^TS) compared to control flies fed with PQ (n = 80) or mock solution (n = 40).

Lifespan and survival curves represent one of two to three independent experiments. Statistical significance was determined by log rank test; ^∗∗p < 0.01, ^∗∗∗p < 0.001.

Scale bars: 50 μm.

See also Figure S5.